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KMID : 0355220100350010019
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Journal of Korean Academy Oral Medicine
2010 Volume.35 No. 1 p.19 ~ p.29
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Effect of Chamaecyparis obtusa tree Phytoncide on Candida albicans
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Kang Soo-Kyung
Auh Q-Schick
Chun Yang-Hyun
Hong Jung-Pyo
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Abstract
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Phytoncide, essential oil of trees, has microbicidal, insecticidal, acaricidal, and deodorizing effect. The present study was performed to examine the effect of phytoncide on Candida albicans, which is a commensal colonizer of the mucous membranes but has become an opportunistic pathogen. C. albicans was incubated with or without phytoncide extracted from Hinoki (Chamaecyparis obtusa Sieb. et Zucc.; Japanese cypress) and then changes were observed in its optical density, cell viability and morphology. As concentrations of phytoncide added to the culture medium increased, optical density and cell viability of C. albicans decreased. Minimum inhibitory concentration of phytoncide for C. albicans was observed to be 0.25%, and minimum fungicidal concentration was 0.5%. Numbers of morphologically atypical cells with electron-dense cytoplasm and granules and increased with increasing concentration of the phytoncide. At higher concentrations of phytoncide, compartments and organelles in the cytoplasm became indistinguishable. The overall results indicate that the phytoncide used for this study has a strong antimicrobial activity against C. albicans. Therefore, the phytoncide may be used as a candidate for prevention and therapeutic agent against oral candidiasis.
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KEYWORD
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Phytoncide, Candida albicans, Minimum Inhibitory Concentration, Minimum Fungicidal Concentration
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